|
週次 |
日期 |
單元主題 |
|
第1週 |
3/05 |
Overview |
|
第2週 |
3/12 |
Generation of Targets for Spotted Microarrays - miniprep and run DNA gel electrophoresis |
|
第3週 |
3/19 |
Generation of Targets for Spotted Microarrays - PCR intro, spectrophotometer intro
|
|
第4週 |
3/26 |
Generation of Targets for Spotted Microarrays - Plate transfer, FX intro |
|
第5週 |
4/02 |
Holiday |
|
第6週 |
4/09 |
Printing Spotted Glass Microarrays - Printing design (OmniGrid intro) (基因體中心7F)
|
|
第7週 |
4/16 |
Printing Spotted Glass Microarrays - Post-processing
|
|
第8週 |
4/23 |
Midterm |
|
第9週 |
4/30 |
Expression Analysis of RNA - Cell culture and RNA extraction |
|
第10週 |
5/07 |
Expression Analysis of RNA - RNA purification and quality check |
|
第11週 |
5/14 |
Expression Analysis of RNA - cDNA labeling
|
|
第12週 |
5/21 |
Expression Analysis of RNA - Hybridization
|
|
第13週 |
5/28 |
Microarray Bioinformatics - Gal file preparation and Scan image (Axon scanner intro) |
|
第14週 |
6/04 |
Microarray Bioinformatics - Web-based Microarray Analysis and IPA intro
|
|
第15週 |
6/11 |
Microarray Bioinformatics - Real-time RT PCR
|
|
第16週 |
6/18 |
Final Exam |